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pcdna3 1 tsmod  (Addgene inc)


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    Addgene inc pcdna3 1 tsmod
    Pcdna3 1 Tsmod, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tsmod+(26021)/TS+module+(Plasmid+%2326021)/pm38986612-586-18-19
    Average 93 stars, based on 13 article reviews
    pcdna3 1 tsmod - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Detection of fluorescent protein mechanical switching in cellulo.
    Article Snippet: .. F-tractin and Linker 1 were derived from pEGFP-C1 F-tractin-EGFP (Addgene Plasmid #58473).32 The original TSMod was derived from pcDNA3.1-TSMod (Addgene Plasmid #26021).30 Linker 2 (GGSGSDPPVAT) was used previously in another construct containing F-tractin.33 Gibson Assembly (with Gibson Assembly Master Mix, E2611S; NEB, Ipswich, MA) was used to generate pcDNA3.1-ABDTS from pcDNA3.1 vector digested with EcoRI-HF (Cat #: R3101S; NEB)/NotI-HF (Cat #: R3189S; NEB) and the following three fragments containing complementary regions: (1) F-tractin and Linker 1, (2) TSMod, and (3) Linker 2 and F-tractin. ..

    Article Title: Detection of Fluorescent Protein Mechanical Switching in Cellulo
    Article Snippet: F-tractin and Linker 1 were derived from pEGFP-C1 F-tractin-EGFP (Addgene Plasmid #5847) . .. The original TSMod was derived from pcDNA3.1-TSMod (Addgene Plasmid #26021) . ..

    Article Title: Detection of fluorescent protein mechanical switching in cellulo
    Article Snippet: F-tractin and Linker 1 were derived from pEGFP-C1 F-tractin-EGFP (Addgene Plasmid #58473). .. The original TSMod was derived from pcDNA3.1-TSMod (Addgene Plasmid #26021). ..

    Plasmid Preparation:

    Article Title: Detection of fluorescent protein mechanical switching in cellulo.
    Article Snippet: .. F-tractin and Linker 1 were derived from pEGFP-C1 F-tractin-EGFP (Addgene Plasmid #58473).32 The original TSMod was derived from pcDNA3.1-TSMod (Addgene Plasmid #26021).30 Linker 2 (GGSGSDPPVAT) was used previously in another construct containing F-tractin.33 Gibson Assembly (with Gibson Assembly Master Mix, E2611S; NEB, Ipswich, MA) was used to generate pcDNA3.1-ABDTS from pcDNA3.1 vector digested with EcoRI-HF (Cat #: R3101S; NEB)/NotI-HF (Cat #: R3189S; NEB) and the following three fragments containing complementary regions: (1) F-tractin and Linker 1, (2) TSMod, and (3) Linker 2 and F-tractin. ..

    Article Title: Förster resonance energy transfer efficiency of the vinculin tension sensor in cultured primary cortical neuronal growth cones
    Article Snippet: The transfection was achieved by one hour incubation of the neurons in each well of a 12 well plate in complete Neurobasal medium containing 1.5 - μ g deoxyribonucleic acid (DNA) plasmid, 1.5 - μ L PLUS reagent, and 2 - μ L Lipofectamine LTX (Invitrogen). .. VinTS, VinTL, and TSMod plasmids were a gift from Martin Schwartz; VinculinTS (Addgene plasmid #26019; RRID:Addgene_26019) VinTL (Addgene plasmid #26020; RRID:Addgene_26020) and TSMod (Addgene plasmid #26021 RRID:Addgene_26021). .. For treatment with the Rho-associated kinase (ROCK) inhibitor Y-27632, the aqueous stock solution (5 mM) of Y-27632 (Cat. #688001, Sigma-Aldrich, St. Louis, Missouri, United States) was dissolved in full Neurobasal medium to a final concentration of 10 - μ M . Neurons expressing VinTS or VinTL were incubated at 37°C and 5% CO 2 for 2 h in full neurobasal medium containing either 10 - μ M Y-27632 or sterile water (vehicle control).

    Article Title: Linear Behavior of the Phase Lifetime in Frequency-Domain Fluorescence Lifetime Imaging of FRET Constructs
    Article Snippet: .. TSMod was obtained from Addgene (TS module Plasmid # 26021). ..

    Article Title: Detection of Fluorescent Protein Mechanical Switching in Cellulo
    Article Snippet: F-tractin and Linker 1 were derived from pEGFP-C1 F-tractin-EGFP (Addgene Plasmid #5847) . .. The original TSMod was derived from pcDNA3.1-TSMod (Addgene Plasmid #26021) . ..

    Article Title: Detection of fluorescent protein mechanical switching in cellulo
    Article Snippet: F-tractin and Linker 1 were derived from pEGFP-C1 F-tractin-EGFP (Addgene Plasmid #58473). .. The original TSMod was derived from pcDNA3.1-TSMod (Addgene Plasmid #26021). ..

    Construct:

    Article Title: Detection of fluorescent protein mechanical switching in cellulo.
    Article Snippet: .. F-tractin and Linker 1 were derived from pEGFP-C1 F-tractin-EGFP (Addgene Plasmid #58473).32 The original TSMod was derived from pcDNA3.1-TSMod (Addgene Plasmid #26021).30 Linker 2 (GGSGSDPPVAT) was used previously in another construct containing F-tractin.33 Gibson Assembly (with Gibson Assembly Master Mix, E2611S; NEB, Ipswich, MA) was used to generate pcDNA3.1-ABDTS from pcDNA3.1 vector digested with EcoRI-HF (Cat #: R3101S; NEB)/NotI-HF (Cat #: R3189S; NEB) and the following three fragments containing complementary regions: (1) F-tractin and Linker 1, (2) TSMod, and (3) Linker 2 and F-tractin. ..

    Article Title: FRET efficiency measurement in a molecular tension probe with a low-cost frequency-domain fluorescence lifetime imaging microscope
    Article Snippet: .. The mTFP 1 - ( GGSGGS ) 2 - mVenus and mTFP1-TRAF-mVenus constructs have been described previously in Ref. . VinTS and TSMod (Plasmids 26019 and 26021, respectively) were obtained from Addgene. ..



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    pcdna3 1 tsmod  (Addgene inc)
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    Addgene inc pcdna3 1 tsmod
    Pcdna3 1 Tsmod, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tsmod  (Addgene inc)
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    (a) Box and whisker plot of FRET efficiency measurements in growth cones from neurons expressing VinTS and <t>TSMod.</t> *** denotes significance of p < 0.001 by Welch’s t -test. The cross (×) denotes the mean. The line denotes the median of the dataset, and the top and bottom of the box denote the 75th and 25th percentile, respectively. The black lines extending from the box (whiskers) corresponds to the maximum and minimum values, which are not considered outliers. Individual points above and below the whiskers are considered outliers with values larger than ± 1.5 × [the difference between the 75th and 25th percentile]. (b) The experiment in (a) was repeated to <t>include</t> <t>VinTL</t> as an additional control. *** denotes significance of p < 0.001 by ANOVA followed by Tukey’s multiple comparisons test. (c) Cumulative distribution functions for pixels from growth cones from neurons expressing VinTS and VinTL [data from (b) only].
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    tsmod coding sequence  (Addgene inc)
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    (a) Box and whisker plot of FRET efficiency measurements in growth cones from neurons expressing VinTS and <t>TSMod.</t> *** denotes significance of p < 0.001 by Welch’s t -test. The cross (×) denotes the mean. The line denotes the median of the dataset, and the top and bottom of the box denote the 75th and 25th percentile, respectively. The black lines extending from the box (whiskers) corresponds to the maximum and minimum values, which are not considered outliers. Individual points above and below the whiskers are considered outliers with values larger than ± 1.5 × [the difference between the 75th and 25th percentile]. (b) The experiment in (a) was repeated to <t>include</t> <t>VinTL</t> as an additional control. *** denotes significance of p < 0.001 by ANOVA followed by Tukey’s multiple comparisons test. (c) Cumulative distribution functions for pixels from growth cones from neurons expressing VinTS and VinTL [data from (b) only].
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    Image Search Results


    (a) Box and whisker plot of FRET efficiency measurements in growth cones from neurons expressing VinTS and TSMod. *** denotes significance of p < 0.001 by Welch’s t -test. The cross (×) denotes the mean. The line denotes the median of the dataset, and the top and bottom of the box denote the 75th and 25th percentile, respectively. The black lines extending from the box (whiskers) corresponds to the maximum and minimum values, which are not considered outliers. Individual points above and below the whiskers are considered outliers with values larger than ± 1.5 × [the difference between the 75th and 25th percentile]. (b) The experiment in (a) was repeated to include VinTL as an additional control. *** denotes significance of p < 0.001 by ANOVA followed by Tukey’s multiple comparisons test. (c) Cumulative distribution functions for pixels from growth cones from neurons expressing VinTS and VinTL [data from (b) only].

    Journal: Neurophotonics

    Article Title: Förster resonance energy transfer efficiency of the vinculin tension sensor in cultured primary cortical neuronal growth cones

    doi: 10.1117/1.NPh.9.2.025002

    Figure Lengend Snippet: (a) Box and whisker plot of FRET efficiency measurements in growth cones from neurons expressing VinTS and TSMod. *** denotes significance of p < 0.001 by Welch’s t -test. The cross (×) denotes the mean. The line denotes the median of the dataset, and the top and bottom of the box denote the 75th and 25th percentile, respectively. The black lines extending from the box (whiskers) corresponds to the maximum and minimum values, which are not considered outliers. Individual points above and below the whiskers are considered outliers with values larger than ± 1.5 × [the difference between the 75th and 25th percentile]. (b) The experiment in (a) was repeated to include VinTL as an additional control. *** denotes significance of p < 0.001 by ANOVA followed by Tukey’s multiple comparisons test. (c) Cumulative distribution functions for pixels from growth cones from neurons expressing VinTS and VinTL [data from (b) only].

    Article Snippet: VinTS, VinTL, and TSMod plasmids were a gift from Martin Schwartz; VinculinTS (Addgene plasmid #26019; RRID:Addgene_26019) VinTL (Addgene plasmid #26020; RRID:Addgene_26020) and TSMod (Addgene plasmid #26021 RRID:Addgene_26021).

    Techniques: Whisker Assay, Expressing